Permeabilization of Bacillus subtilis to chemotaxis methyltransferase II
نویسندگان
چکیده
منابع مشابه
Permeabilization of Bacillus subtilis to Chemotaxis
The net methylation of methyl-accepting chemotaxis proteins (MCPs) is the biochemical manifestation of physiological adaptation to amino acid chemoattractants by Escherichia coli (4). In Bacillus subtilis, demethylation of MCPs occurs during such adaptation (3). The activity of chemotaxis methyltransferase is detectable in vivo and in vitro. As an alternative assay, whole cells can be permeabil...
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A Bacillus subtilis methyltransferase capable of methylating membrane-bound methyl-accepting chemotaxis proteins (MCPs) of a chemotaxis mutant was purified to homogeneity. MCPs are normally unmethylated in this strain. Results of gel filtration chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicate that the enzyme is a 30,000 molecular weight monomer. The nzym...
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The Bacillus subtilis chemotaxis pathway employs a receptor methylation system that functions differently from the one in the canonical Escherichia coli pathway. Previously, we hypothesized that B. subtilis employs a site-specific methylation system for adaptation where methyl groups are added and removed at different sites. This study investigated how covalent modifications to the adaptation r...
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Adaptation has a crucial role in the gradient-sensing mechanism that underlies bacterial chemotaxis. The Escherichia coli chemotaxis pathway uses a single adaptation system involving reversible receptor methylation. In Bacillus subtilis, the chemotaxis pathway seems to use three adaptation systems. One involves reversible receptor methylation, although quite differently than in E. coli. The oth...
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Bacillus subtilis, like Escherichia coli and Salmonella typhimurium, carries out chemotaxis by modulating the relative frequency of smooth swimming and tumbling. Like these enteric bacteria, methionine auxotrophs starved for methionine show an abnormally long-period of smooth swimming after addition of attractant. This "hypersensitive" state requires an hour of starvation for its genesis, which...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1983
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.155.2.907-909.1983